The long-term aim of this research is to investigate the properties of cholesterol bilayer domains (CBDs). Before starting experimental work, we conducted a detailed literature search and wrote a review article about all membrane models and experimental methods suitable for studying properties of CBD domains. We also described in details their known properties and functions in biological membranes. Our motivation stemmed from the beneficial role of CBDs in protecting the eye lens from cataract development and maintaining its homeostasis. Based on this knowledge we chosen giant unilamellar vesicles (GUVs) as an appropriate model for studying lipid portion of the fiber cell plasma membranes of the eye lens. GUVs are widely used as membrane models in studies of membrane properties, with the electroformation method being the most common approach for their production. However, several factors can affect the success of GUV electroformation. Common challenges include high cholesterol (Chol) concentrations, the use of lipid mixtures containing charged lipids, and solutions with elevated ionic strength. High Chol concentrations pose a particular problem for the traditional electroformation protocol, which involves drying the lipid film by fully evaporating the organic solvent. During this process, anhydrous Chol crystals can form, which do not integrate into the lipid bilayer, resulting in a lower Chol concentration in the vesicle bilayer compared to the original lipid mixture. To address the issue of artifactual Chol demixing, we have modified the electroformation protocol by introducing rapid solvent exchange, ultrasonication, plasma cleaning, and spin-coating techniques for the reproducible production of GUVs from damp lipid films. A high yield of GUVs was achieved for Chol/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) samples with mixing ratios ranging from 0 to 2.5. At Chol/POPC mixing ratios greater than 1,5, where the GUVs may contain Chol bilayer domains (CBDs), a significant decrease in the average GUV diameter is measured. It is about 40% lower compared to that of the pure POPC bilayer. This modified approach not only minimizes Chol demixing but also successfully produces GUVs from lipid mixtures containing charged lipids and ionic solutions as the internal medium. We prepared GUVs from mixtures with up to 60 mol% of the charged lipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS) and formed GUVs in low-concentration NaCl solutions.