prikaz prve stranice dokumenta Strucural studies, antimicrobial activity and toxicity of antimicrobial peptides identifed in tapeworm (Taenia solium Linnaeus, 1758)
Embargo period 2027-09-30
master's thesis
Strucural studies, antimicrobial activity and toxicity of antimicrobial peptides identifed in tapeworm (Taenia solium Linnaeus, 1758)
2024. urn:nbn:hr:166:563802
Bilandžić, Katijana
University of Split
Faculty of Science

Cite this document

Bilandžić, K. (2024). Strucural studies, antimicrobial activity and toxicity of antimicrobial peptides identifed in tapeworm (Taenia solium Linnaeus, 1758) (Master's thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:563802

Bilandžić, Katijana. "Strucural studies, antimicrobial activity and toxicity of antimicrobial peptides identifed in tapeworm (Taenia solium Linnaeus, 1758)." Master's thesis, University of Split, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:166:563802

Bilandžić, Katijana. "Strucural studies, antimicrobial activity and toxicity of antimicrobial peptides identifed in tapeworm (Taenia solium Linnaeus, 1758)." Master's thesis, University of Split, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:166:563802

Bilandžić, K. (2024). 'Strucural studies, antimicrobial activity and toxicity of antimicrobial peptides identifed in tapeworm (Taenia solium Linnaeus, 1758)', Master's thesis, University of Split, Faculty of Science, accessed 31 October 2024, https://urn.nsk.hr/urn:nbn:hr:166:563802

Bilandžić K. Strucural studies, antimicrobial activity and toxicity of antimicrobial peptides identifed in tapeworm (Taenia solium Linnaeus, 1758) [Master's thesis]. Split: University of Split, Faculty of Science; 2024 [cited 2024 October 31] Available at: https://urn.nsk.hr/urn:nbn:hr:166:563802

K. Bilandžić, "Strucural studies, antimicrobial activity and toxicity of antimicrobial peptides identifed in tapeworm (Taenia solium Linnaeus, 1758)", Master's thesis, University of Split, Faculty of Science, Split, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:166:563802

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Metadata
TitleStrucural studies, antimicrobial activity and toxicity of antimicrobial peptides identifed in tapeworm (Taenia solium Linnaeus, 1758)
Title (croatian)Strukturalna karakterizacija, antimikrobna aktivnost i toksičnost antimikrobnih peptida identificiranih u trakavice (Taenia solium Linnaeus, 1758)
AuthorKatijana Bilandžić
MentorTomislav Rončević (mentor)
MentorAlessandro Tossi https://orcid.org/0000-0001-9087-5764 (pomoć pri izradi, neposredni voditelj)
MentorSabrina Pacor https://orcid.org/0000-0001-8043-6725 (pomoć pri izradi, neposredni voditelj)
Committee memberTomislav Rončević (predsjednik povjerenstva)
Committee memberŽeljana Fredotović (član povjerenstva)
Committee memberIvica Šamanić (član povjerenstva)
GranterUniversity of Split
Faculty of Science
Split
Defense date and country2024-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
All living organisms are constantly threatened by large number and great diversity of pathogens which is why all of them synthesize antimicrobial peptides (AMPs) as a first line of defense as a part of their innate immunity. These molecules show direct antimicrobial and/or immunomodulatory properties which make them a potential source of new therapeutics during global antimicrobial resistance crisis. As one of the most successful animal groups in nature, parasitic helminths (worms) found different ways to overcome and avoid expulsion by their host’s immune system. One of them is by secreting different immunomodulatory molecules one of which are also AMPs. Helminth’s coevolution with the host gut microbiome and reciprocal adaptation which favors development of compounds minimally invasive to the host make helminthic AMPs especially interesting for further investigation. The aim of this thesis was to characterize three potential AMPs from tapeworm (Taenia solium). That included bioinformatical analysis, structural characterization by CD spectroscopy, determination of antimicrobial activity and subsequent mode of action by propidium iodide (PI) uptake assay using flow cytometry. Cytotoxic activity was tested with MTT assay and late confirmed with PI uptake assay using flow cytometry. Two out of three peptides showed potent antibacterial activity with borderline levels of toxicity which should be addressed if the peptides undergo further studies for potential therapeutical applications.
Abstract (croatian)
Svi živi organizmi konstantno su ugroženi velikim brojem i velikom raznolikošću patogena, zbog čega sintetiziraju antimikrobne peptide (AMP) kao prvu liniju obrane i dio svog urođenog imunološkog odgovora. Ove molekule pokazuju izravno antimikrobno i/ili imunomodulacijsko djelovanje što ih čini potencijalnim izvorom novih lijekova tijekom globalne krize razvoja rezistencije na antibiotike. Kao jedna od najuspješnijih životinjskih skupina u prirodi, parazitski crvi su pronašli različite načine da nadvladaju i izbjegnu imunološki sustav svog domaćina. Jedan od njih je izlučivanje različitih imunomodulacijskih molekula kao što su npr. antimikrobni peptidi. Koevolucija parazitskih crva s crijevnim mikrobiomom domaćina i recipročna prilagodba koja pogoduje sekreciji minimalno invazivnih spojeva čini ove antimikrobne peptide posebno zanimljivima za daljnje istraživanje. Cilj ovog rada bio je istražiti biološku aktivnost tri potencijalna AMP-a iz trakavice, Taenia solium. To je uključivalo bioinformatičku analizu, strukturnu karakterizaciju CD spektroskopijom, određivanje antimikrobne aktivnosti i mehanizma djelovanja koristeći propidij jodid i protočnu citometriju. Citotoksična aktivnost ispitana je MTT testovima i kasnije potvrđena koristeći propidij jodid (PI) i protočnu citometriju . Dva od tri peptida pokazala su potencijalno antibakterijsko djelovanje s graničnim razinama toksičnosti koju bi trebalo poboljšati ukoliko se peptidi budu dalje istraživali u svrhu potencijalne terapijske primjene.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:166:563802
Study programmeTitle: Biology and Chemistry; specializations in: specialization in Education
Course: specialization in Education
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra edukacije biologije i kemije (sveučilišni/a magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2027-09-30
Terms of useLicence In copyright icon
Created on2024-10-29 12:30:58