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undergraduate thesis
Jednostavna priprema stanica za istraživanje pretražnim elektronskim mikroskopom
2023. urn:nbn:hr:166:600714
Perković, Luka
University of Split
Faculty of Science
Department of Physics

Cite this document

Perković, L. (2023). Jednostavna priprema stanica za istraživanje pretražnim elektronskim mikroskopom (Undergraduate thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:600714

Perković, Luka. "Jednostavna priprema stanica za istraživanje pretražnim elektronskim mikroskopom." Undergraduate thesis, University of Split, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:166:600714

Perković, Luka. "Jednostavna priprema stanica za istraživanje pretražnim elektronskim mikroskopom." Undergraduate thesis, University of Split, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:166:600714

Perković, L. (2023). 'Jednostavna priprema stanica za istraživanje pretražnim elektronskim mikroskopom', Undergraduate thesis, University of Split, Faculty of Science, accessed 23 April 2024, https://urn.nsk.hr/urn:nbn:hr:166:600714

Perković L. Jednostavna priprema stanica za istraživanje pretražnim elektronskim mikroskopom [Undergraduate thesis]. Split: University of Split, Faculty of Science; 2023 [cited 2024 April 23] Available at: https://urn.nsk.hr/urn:nbn:hr:166:600714

L. Perković, "Jednostavna priprema stanica za istraživanje pretražnim elektronskim mikroskopom", Undergraduate thesis, University of Split, Faculty of Science, Split, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:166:600714

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Metadata
TitleJednostavna priprema stanica za istraživanje pretražnim elektronskim mikroskopom
Title (english)Simple preparation of cells for scanning electron microscope research
AuthorLuka Perković
MentorLucija Krce (mentor)
Committee memberLucija Krce (predsjednik povjerenstva)
Committee memberAnte Bilušić (član povjerenstva)
Committee memberMatilda Šprung (član povjerenstva)
GranterUniversity of Split
Faculty of Science
(Department of Physics)
Split
Defense date and country2023-07-20, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract
Cilj ovog eksperimentalnog rada je jednostavnom, dostupnom i brzom metodom pripremiti stanice za pretražnu elektronsku mikroskopiju te istražiti njihovu morfologiju. U eksperimentu su korištene stanice sisavaca koje su uzgojene na vodljivoj podlozi, fiksirane glutaraldehidom, dehidrirane etanolom i osušene na zraku. Budući da su stanice nevodljive, na njih je, neposredno prije mikroskopiranja, nanesen nano-sloj metala magnetronskim rasprašivačem. U radu je opisan princip rada pretražnog elektronskog mikroskopa i proces nastajanja mikrografa. Objašnjena je priprema različitih stanica i opisana njihova morfologija. Uspješnim oslikavanjem je pokazano da je ova jednostavna i brza metoda pripreme stanica dovoljno dobra za istraživanje njihove morfologije pretražnim elektronskim mikroskopom.
Abstract (english)
The aim of this experimental work is to prepare cells for scanning electron microscopy using a simple, available, and fast method and to study their morphology. Mammalian cells grown on conductive surfaces, fixed with glutaraldehyde, dehydrated with ethanol and air dried were used for the experiment. Since the cells are not conductive, a nano-metallic layer was applied to the cells by magnetron sputtering immediately before microscopy. The thesis describes the working principle of the scanning electron microscope and the process of creating micrographs. The preparation of the different cells is explained, and their morphology is described. Successful imaging has shown that this simple and fast method of cell preparation is suitable to study their morphology with a scanning electron microscope.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:166:600714
Study programmeTitle: Physics
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) fizike (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) fizike)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2023-07-21 10:48:53