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master's thesis
Identifikacija plazmida koji kodiraju karbapenemazu KPC-2 kod Enterobacteriaceae iz okoliša
2022. urn:nbn:hr:166:225663
Bellulovich, Ema
University of Split
Faculty of Science
Department of Biology

Cite this document

Bellulovich, E. (2022). Identifikacija plazmida koji kodiraju karbapenemazu KPC-2 kod Enterobacteriaceae iz okoliša (Master's thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:225663

Bellulovich, Ema. "Identifikacija plazmida koji kodiraju karbapenemazu KPC-2 kod Enterobacteriaceae iz okoliša." Master's thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:225663

Bellulovich, Ema. "Identifikacija plazmida koji kodiraju karbapenemazu KPC-2 kod Enterobacteriaceae iz okoliša." Master's thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:225663

Bellulovich, E. (2022). 'Identifikacija plazmida koji kodiraju karbapenemazu KPC-2 kod Enterobacteriaceae iz okoliša', Master's thesis, University of Split, Faculty of Science, accessed 12 December 2024, https://urn.nsk.hr/urn:nbn:hr:166:225663

Bellulovich E. Identifikacija plazmida koji kodiraju karbapenemazu KPC-2 kod Enterobacteriaceae iz okoliša [Master's thesis]. Split: University of Split, Faculty of Science; 2022 [cited 2024 December 12] Available at: https://urn.nsk.hr/urn:nbn:hr:166:225663

E. Bellulovich, "Identifikacija plazmida koji kodiraju karbapenemazu KPC-2 kod Enterobacteriaceae iz okoliša", Master's thesis, University of Split, Faculty of Science, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:166:225663

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Metadata
TitleIdentifikacija plazmida koji kodiraju karbapenemazu KPC-2 kod Enterobacteriaceae iz okoliša
Title (english)Identification of carbapenemase KPC-2 encoding plasmids among environmental Enterobacteriaceae
AuthorEma Bellulovich
MentorIvica Šamanić (mentor)
Committee memberIvica Šamanić (predsjednik povjerenstva)
Committee memberAna Maravić (član povjerenstva)
Committee memberMia Dželalija (član povjerenstva)
GranterUniversity of Split
Faculty of Science
(Department of Biology)
Split
Defense date and country2022-09-06, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Pojava rezistentnih sojeva Enterobacteriaceae posljedica je nerijetke uporabe karbapenemskih antibiotika u kliničkoj praksi. Karbapenemaze hidroliziraju karbapenemske antibiotike, a geni koji ih kodiraju su povezani s visokorizičnim klonovima i šire se horizontalnim prijenosom pomoću plazmida. Cilj ovog istraživanja bio je identificirati plazmide koji kodiraju KPC-2 kod Enterobacteriaceae iz okoliša. Od 30 izolata u kojima je sekvencioniranjem utvrđena prisutnost \(bla_{KPC}\) gena u njih 18 utvrđen je njihov položaj na plazmidima. Plazmidi koji kodiraju \(bla_{KPC-2}\) gen veličine su od 33.3 kb do 160 kb, među kojima su neki od njih već prethodno identificirani u bolničkom okruženju te otpadnim vodama u Hrvatskoj što ukazuje na njihovu diseminaciju u prirodni okoliš. Bitno je također istaknuti da KPC-producirajući sojevi nisu do sada izolirani iz morske vode s javne plaže u Hrvatskoj što ukazuje na mogući javnozdravstveni problem olakšanog širenja među ljudskom populacijom i potrebu za daljnjim istraživanjem njihove rasprostranjenosti u okolišu.
Abstract (english)
The emergence of resistant Enterobacteriaceae strains is a consequence of the not so rare use of carbapenems in clinical practice. Carbapenemases hydrolyse carbapenems, while KPC encoding genes are associated with high-risk clones and spread through horizontal transmission. The aim of this study was to identify the plasmids encoding the KPC-2 in environmental Enterobacteriaceae. In 18 out of 30 \(bla_{KPC}\)-positive isolates their position on plasmids was determined. Plasmids encoding the \(bla_{KPC-2}\) gene range from 33.3 kb to 160 kb, among which some of them have already been identified in the hospital environment and wastewaters in Croatia, indicating their dissemination into the natural environment. It is important to note that KPC-producing strains have not yet been isolated from seawater from a public beach in Croatia which may present a public health risk due to easier spreading among the human population, as well as the need for further investigation of their distribution in the environment.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:166:225663
ProjectNumber: UIP-2019-04-9778
Title: Sezonska i prostorna raspodjela gena rezistencije na antibiotike u morskim mikrobnim zajednicama duž trofičkog gradijenta u srednjem Jadranu
Title: Seasonal and spatial distribution of antibiotic resistance genes in marine microbial communities along a trophic gradient in central Adriatic Sea
Acronym: ARGAS
Leader: Ana Maravić
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: UIP
Study programmeTitle: Biology and Chemistry; specializations in: major in teaching
Course: major in teaching
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-09-07 12:34:17