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doctoral thesis
Experimental Investigation and Modelling of Bacterial Growth and Inactivation: E. coli Exposed to Laser-synthesized Silver Nanoparticles
2020. urn:nbn:hr:166:803690
Krce, Lucija
University of Split
Faculty of Science
Department of Physics

Cite this document

Krce, L. (2020). Experimental Investigation and Modelling of Bacterial Growth and Inactivation: E. coli Exposed to Laser-synthesized Silver Nanoparticles (Doctoral thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:803690

Krce, Lucija. "Experimental Investigation and Modelling of Bacterial Growth and Inactivation: E. coli Exposed to Laser-synthesized Silver Nanoparticles." Doctoral thesis, University of Split, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:166:803690

Krce, Lucija. "Experimental Investigation and Modelling of Bacterial Growth and Inactivation: E. coli Exposed to Laser-synthesized Silver Nanoparticles." Doctoral thesis, University of Split, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:166:803690

Krce, L. (2020). 'Experimental Investigation and Modelling of Bacterial Growth and Inactivation: E. coli Exposed to Laser-synthesized Silver Nanoparticles', Doctoral thesis, University of Split, Faculty of Science, accessed 13 November 2024, https://urn.nsk.hr/urn:nbn:hr:166:803690

Krce L. Experimental Investigation and Modelling of Bacterial Growth and Inactivation: E. coli Exposed to Laser-synthesized Silver Nanoparticles [Doctoral thesis]. Split: University of Split, Faculty of Science; 2020 [cited 2024 November 13] Available at: https://urn.nsk.hr/urn:nbn:hr:166:803690

L. Krce, "Experimental Investigation and Modelling of Bacterial Growth and Inactivation: E. coli Exposed to Laser-synthesized Silver Nanoparticles", Doctoral thesis, University of Split, Faculty of Science, Split, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:166:803690

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Metadata
TitleExperimental Investigation and Modelling of Bacterial Growth and Inactivation: E. coli Exposed to Laser-synthesized Silver Nanoparticles
Title (croatian)Eksperimentalno istraživanje i modeliranje bakterijskog rasta i inaktivacije: E. coli izložena laserski sintetiziranim srebrnim nanočesticama
AuthorLucija Krce
MentorIvica Aviani (mentor)
Committee memberAnte Bilušić (predsjednik povjerenstva)
Committee memberNikša Krstulović (član povjerenstva)
Committee memberMarija Raguž (član povjerenstva)
GranterUniversity of Split
Faculty of Science
(Department of Physics)
Split
Defense date and country2020-07-03, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
The aim of this thesis is to elaborate on the mode of antibacterial action by colloidal silver nanoparticles against Escherichia coli as a model organism. Two approaches were used to investigate the bactericidal mechanism of silver nanoparticles (AgNPs): experimental probing and modelling of the treated bacterial cell growth. AgNPs were synthesized by laser ablation in water and the obtained colloid was thoroughly characterized. An upgradable interaction-based growth model was developed to understand the effect of AgNPs on bacterial cell dynamics. The model explains untreated and AgNP-treated Escherichia coli growth in batch culture. Subsequently, fluorescence probing and atomic force microscopy and spectroscopy were used to experimentally study the AgNP bactericidal mechanism. This study shows that the mode of antibacterial action of silver nanoparticles includes penetration of AgNPs inside the cell/membrane, membrane permeabilization, reduction of the cell’s Young modulus and possible cell leakage while undistinguished reactive oxygen species level was detected inside lethally treated cells.
Abstract (croatian)
Cilj ove teze je razjasniti način antibakterijskog djelovanja koloidnih nanočestica srebra na stanice Escherichia coli kao modelnog organizma. Istraživanje baktericidnog mehanizma srebrnih nanočestica provedeno je eksperimentalnim i teorijskim metodama kako bi se čim bolje opisao rast tretiranih bakterijskih stanica. Srebrne nanočestice su sintetizirane laserskom ablacijom u vodi, a dobiveni koloid je temeljito karakteriziran. Uveden je model rasta temeljen na interakcijama kako bi se razumio učinak nanočestica na dinamiku rasta bakterijskih stanica. Model objašnjava rast netretiranih i tretiranih bakterijskih stanica u tekućoj kulturi. Za eksperimentalno proučavanje baktericidnog mehanizma nanočestica korištene su fluorescentna spektroskopija te mikroskopija i spektroskopija atomskom silom. Istraživanje pokazuje da antibakterijsko djelovanje srebrnih nanočestica na bakterijske stanice uključuje njihov prodor unutar stanice/membrane, permeabilizaciju membrane, smanjenje Youngovog modula s vremenom te moguće istjecanje unutarstaničnog materijala, dok je inducirana razina reaktivnih kisikovih vrsta u smrtonosno tretiranim stanicama zanemariva.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:166:803690
Study programmeTitle: Postgraduate University Study of Biophysics
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje fizika (doktor/doktorica znanosti, područje prirodnih znanosti, polje fizika)
Type of resourceText
Extent97 str.; 30 cm
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-07-24 12:30:50