Creating a map and listing of the plants in the Split botanical garden was conducted during 2015 and 2016 and an area of 2 acres within the park forest Marjan was covered. Marjan Botanical Garden of the Faculty of Science, University of Split, shows wide range of wild and cultivated plants. Botanical garden has a total of about 500 different plant species, of which 355 plants grow in open areas of the garden, and the rest in the greenhouses. The most represented families in the flora of the garden are Lamiaceae with 30 species and family Asteraceae with 27 species. The most frequent life forms are phanerophytes with 146 species (41.13%), followed by therophytes with 26 species (7.32%), and chamaephytes with 42 species (11.83%). Most dominating floral element is the Mediterranean floral element with 156 (44%) plant species. Number of cultivated and adventitious plants (116-33%) is expectedly high, which indicates that there is the antrophogenic influence in the garden. There are many endemic, endangered and strictly protected plant species in the garden and for that it is of the outmost importance.