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master's thesis
Tehnologija CRISPR-Cas9: Načela i primjene u genetičkom inženjerstvu
2019. urn:nbn:hr:166:915255
Kukolj, Matea
University of Split
University of Split, Faculty of science
Department of Physics

Cite this document

Kukolj, M. (2019). Tehnologija CRISPR-Cas9: Načela i primjene u genetičkom inženjerstvu (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:915255

Kukolj, Matea. "Tehnologija CRISPR-Cas9: Načela i primjene u genetičkom inženjerstvu." Master's thesis, University of Split, University of Split, Faculty of science, 2019. https://urn.nsk.hr/urn:nbn:hr:166:915255

Kukolj, Matea. "Tehnologija CRISPR-Cas9: Načela i primjene u genetičkom inženjerstvu." Master's thesis, University of Split, University of Split, Faculty of science, 2019. https://urn.nsk.hr/urn:nbn:hr:166:915255

Kukolj, M. (2019). 'Tehnologija CRISPR-Cas9: Načela i primjene u genetičkom inženjerstvu', Master's thesis, University of Split, University of Split, Faculty of science, accessed 24 May 2022, https://urn.nsk.hr/urn:nbn:hr:166:915255

Kukolj M. Tehnologija CRISPR-Cas9: Načela i primjene u genetičkom inženjerstvu [Master's thesis]. Split: University of Split, University of Split, Faculty of science; 2019 [cited 2022 May 24] Available at: https://urn.nsk.hr/urn:nbn:hr:166:915255

M. Kukolj, "Tehnologija CRISPR-Cas9: Načela i primjene u genetičkom inženjerstvu", Master's thesis, University of Split, University of Split, Faculty of science, Split, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:166:915255

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Metadata
TitleTehnologija CRISPR-Cas9: Načela i primjene u genetičkom inženjerstvu
Title (english)CRISPR-Cas9 technology: principles and applications in genetic engineering
AuthorMatea Kukolj
MentorJasna Puizina (mentor)
Committee memberJasna Puizina (predsjednik povjerenstva)
Committee memberLarisa Zoranić (član povjerenstva)
Committee memberŽeljka Sanader Maršić (član povjerenstva)
GranterUniversity of Split
University of Split, Faculty of science
(Department of Physics)
Split
Defense date and country2019-10-31, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Interdisciplinary Natural Sciences
Abstract
Specifične kratke palindromske sekvence DNA nazvane CRISPR („Clustered, Regularly Interspaced Short Palindromic Repeats“) pronađene su u gotovo svim arhebakterijama i dijelu eubakterija, a kroz proteklih 20 godina proučavana je njihova uloga i moguća primjena. S tim sekvencama povezan protein kaspaza 9 (Cas9), endonukleaza, koja se vođena kratkom molekulom RNA (vodič, engl. guide RNA, gRNA) veže uz ciljnu molekulu DNA i potom ju cijepa. Prenosivost kaspaze 9 među vrstama omogućila je brojne primjene sustava CRISPR-Cas9 u genetičkom inženjerstvu. Programiranje sustava CRISPRCas9 u laboratoriju u pravilu se sastoji od dizajniranja vodič RNA i odabira popravka izazvanog dvolančanog loma DNA, a moguće je i izazivanje mutacija u domenama kaspaze 9 sa svrhom promjena u specifičnosti sustava. Razvoj tehnika poput mikroskopije atomskih sila (AFM) od ključne su važnosti za vizualizaciju ponašanja sustava CRISPR-Cas9 u stvarnom vremenu. Do sada su tehnologijom CRISPR-Cas9 genetički modificirani brojni usjevi, ali postoje i brojna istraživanja učinkovitosti tehnologije kod bolesti ljudi. Usavršavanje tehnike CRISPR-Cas9 ključno je za provođenje kliničkih ispitivanja, koja će biti od iznimne važnosti za liječenje širokog spektra bolesti u ljudi.
Abstract (english)
CRISPR (“Clustered, Regularly Interspaced Short Palindromic Repeats”) sequences were found in almost all archaea and partially in bacteria and over past 20 years their role and possible applications have been studied. Protein Cas9, that is connected with those sequences, is a nuclease that, guided by short RNA molecule (guide RNA, gRNA) binds to the target DNA molecule and cleaves it. Transferability of Cas9 between the species has allowed multiple applications of CRISPR-Cas9 system in genetic engineering. Programmability of CRISPR-Cas9 system includes designing gRNA and choosing the repair pathway for induced double-strand break on DNA, but there are also possibilities like introducing mutations in Cas9 domains in order to influence system specificity. Development of techniques such as atomic force microscopy (AFM) will play a key role in visualization of CRISPRCas9 system in real-time. So far, plenty of fruit crops have been modified using CRISPR-Cas9 technology, but there has also been a lot of research of technology’s efficiency in field of human diseases. Perfecting the CRISPR-Cas9 technique is key for conduction of clinical trials, which will be of great importance in treatment of a wide spectrum of human diseases.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:166:915255
Study programmeTitle: Physics; specializations in: major in teaching, major in Astrophysics, major in Biophysics, major in Enviromental physics, major in Computational physics
Course: major in Biophysics
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra fizike (magistar/magistra fizike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-06 09:18:03