Određivanje antimikrobne rezistencije bakterija porodice Enterobacteriaceae u hrani

Pera, Kristina

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master's thesis

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Određivanje antimikrobne rezistencije bakterija porodice Enterobacteriaceae u hrani

Kristina Pera (2019)

University of Split
University of Split, Faculty of science
Department of Biology

Cite this document

APA 6th Edition

Pera, K. (2019). Određivanje antimikrobne rezistencije bakterija porodice Enterobacteriaceae u hrani (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:568247

MLA 8th Edition

Pera, Kristina. "Određivanje antimikrobne rezistencije bakterija porodice Enterobacteriaceae u hrani." Master's thesis, University of Split, University of Split, Faculty of science, 2019. https://urn.nsk.hr/urn:nbn:hr:166:568247

Chicago 17th Edition

Harvard

Pera, K. (2019). 'Određivanje antimikrobne rezistencije bakterija porodice Enterobacteriaceae u hrani', Master's thesis, University of Split, University of Split, Faculty of science, accessed 20 February 2020, https://urn.nsk.hr/urn:nbn:hr:166:568247

Vancouver

Pera K. Određivanje antimikrobne rezistencije bakterija porodice Enterobacteriaceae u hrani [Master's thesis]. Split: University of Split, University of Split, Faculty of science; 2019 [cited 2020 February 20] Available at: https://urn.nsk.hr/urn:nbn:hr:166:568247

IEEE

K. Pera, "Određivanje antimikrobne rezistencije bakterija porodice Enterobacteriaceae u hrani", Master's thesis, University of Split, University of Split, Faculty of science, Split, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:166:568247

Cite this item: https://urn.nsk.hr/urn:nbn:hr:166:568247

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Metadata

Title	Određivanje antimikrobne rezistencije bakterija porodice Enterobacteriaceae u hrani
Title (english)	Determination of Enterobacteriaceae antimicrobial resistance in food
Author	Kristina Pera
Mentor	Mirjana Skočibušić (mentor)
Committee member	Mirjana Skočibušić (predsjednik povjerenstva)
Committee member	Ivana Bočina (član povjerenstva)
Committee member	Antonela Paladin (član povjerenstva)
Granter	University of Split University of Split, Faculty of science (Department of Biology) Split
Defense date and country	2019-03-25, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Cilj rada je procjena učestalosti bakterija porodice Enterobacteriaceae rezistentnih na antibiotike u različitim namirnicama. Istraživanja su provedena od travnja do lipnja 2018. godine, a ukupno je analizirano 38 namirnica. Izolacija i identifikacija bakterija je provedena standardnim mikrobiološkim metodama. Osjetljivost izolata određena je metodom disk difuzije na 13 antibiotika, dok je produkcija β-laktamaza proširenog spektra (ESBL) utvrđena metodom dvostrukog diska. U istraživanim uzorcima izolirano je šest različitih vrsta bakterija. Najveću učestalost imala je Klebsiella pneumoniae nakon koje slijede Enterobacter aerogenes, E. cloacae, te Escherichia coli i Citrobacter sp. Izolati E. coli bili su rezistentni na ampicilin, tetraciklin, piperacilin, astreonam, te trimetoprim-sulfametoksazol. Izolirane vrste roda Enterobacter ukazuju na visoki stupanj rezistencije. Također, visoka razina rezistencije uočena je kod K. pneumoniae izolata. U izolatima K. pneumoniae dokazano je prisustvo TEM, SHV, CTX tip β-laktamaza. Dobiveni rezultati će pridonijeti boljem razumijevanju uloge hrane kao mogućeg rezervoara rezistentnih bakterija i njihovih gena.
Abstract (english)	The aim of this study was to determine the prevalence of antibiotic resistant bacteria in the Enterobacteriaceae. The research was conducted from April to June 2018, and a total of 38 foods were analyzed. Isolation and identification of different types of Enterobacteriaceae was performed by standard microbiological methods. Sensitivity of the isolates was determined by the disc diffusion method at 13 antibiotics, while the β-lactamase extended spectrum production (ESBL) was determined by the double disk method. Six different species of bacteria were isolated in the investigated samples. The highest frequency had Klebsiella pneumoniae followed by Enterobacter aerogenes, E. cloacae, and Escherichia coli and Citrobacter sp. Isolated E. coli were resistant to ampicillin, tetracycline, piperacillin, astreonam, and trimethoprim-sulfamethoxazole. Isolated species of Enterobacter indicate a high level of resistance. Also, a high level of resistance was observed in K. pneumoniae isolates. In the isolates of K. pneumoniae, the presence of TEM, SHV, CTX type of β-lactamases were demonstrated. The achieved results will contribute to a better understanding the role of food as a potential reservoir of resistant bacteria and their genes.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:166:568247
Study programme	Title: Biology and Chemistry; specializations in: major in teaching Course: major in teaching Study programme type: university Study level: graduate Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
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Created on	2019-10-11 06:50:07