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master's thesis
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Chemical composition and antiviral activity of hydrolate of Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. on tobacco mosaic virus
Nela Mandić (2018)
University of Split
University of Split, Faculty of science
Department of Biology
Metadata
TitleKemijski sastav i protuvirusno djelovanje hidrolata vrste Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. na virus mozaika duhana
AuthorNela Mandić
Mentor(s)Elma Vuko (thesis advisor)
Abstract
Vrsta Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. (Clusiaceae) aromatična je biljna vrsta čija je ljekovitost od davnina poznata u narodnoj medicini. Iz biljnog materijala je izdvojen hidrolat kao nusprodukt vodene destilacije pri izolaciji eteričnog ulja. Analiza kemijskog sastava hidrolata provedena je primjenom tekućinske kromatografije visoke djelotvornosti (HPLC). Najzastupljenije komponente u sastavu hidrolata su 3,4-dihidroksibenzojeva kiselina (22,66µg/mL), apigenin (2,57µg/mL) i pinorezinol (2,46µg/mL). Protuvirusno djelovanje hidrolata na virus mozaika duhana ispitano je na biljkama koje su lokalni i sistemični domaćini ovog virusa. Pred tretman biljaka hidrolatom smanjuje broj virusnih lezija na listovima lokalnih domaćina i koncentraciju virusa u sistemično inficiranim biljkama. Dobiveni rezultati ukazuju da tretman hidrolatom vrste H. perforatum ssp. veronense jača otpornost biljaka prema virusnoj infekciji te da hidrolati aromatičnih biljnih vrsta imaju potencijal za razvoj prirodnih protufitovirusnih preparata.
KeywordsHypericum perforatum ssp. veronense hydrolate 3.4-dihydroxybenzoic acid tobacco mosaic virus antiviral activity
Parallel title (English)Chemical composition and antiviral activity of hydrolate of Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. on tobacco mosaic virus
Committee MembersElma Vuko (committee chairperson)
Valerija Dunkić (committee member)
Mirko Ruščić (committee member)
GranterUniversity of Split
University of Split, Faculty of science
Lower level organizational unitsDepartment of Biology
PlaceSplit
StateCroatia
Scientific field, discipline, subdisciplineNATURAL SCIENCES
Biology
Study programme typeuniversity
Study levelgraduate
Study programmeBiology and Chemistry; specializations in: major in teaching
Study specializationmajor in teaching
Academic title abbreviationmag. educ. biol. et chem.
Genremaster's thesis
Language Croatian
Defense date2018-09-27
Parallel abstract (English)
Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. (Clusiaceae) is an aromatic plant species well known in folk medicine. Hydrolate was isolated from plant material and an analysis of it's chemical composition was carried out using high performance liquid chromatography (HPLC). The main components of the hydrolate are 3,4-dihydroxybenzoic acid (22.66µg/mL), apigenin (2.57µg/mL) and pinorezinol (2.46µg/mL). Antiviralactivity of the hydrolate was tested on plants that are local and systemic hosts of tobacco mosaic virus. Pretreatment by hydrolate reduced the number of lesions on the leaves of local host plants as well as the virus concentration in systemically infected plants. The results indicate that treatment by hydrolate of H. perforatum ssp. veronense increases the plants resistance to viral infection and that hydrolates of aromatic plant species have the potential for development of natural antiphytoviral preparations.
Parallel keywords (Croatian)Hypericum perforatum ssp. veronense hidrolat 3.4-dihidroksibenzojeva kiselina virus mozaika duhana protuvirusno djelovanje
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:166:076828