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Chemical composition and antiviral activity of hydrolate of Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. on tobacco mosaic virus
Nela Mandić (2018)
University of Split
University of Split, Faculty of science
Department of Biology
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Mandić, N. (2018). Kemijski sastav i protuvirusno djelovanje hidrolata vrste Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. na virus mozaika duhana (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:076828

Mandić, Nela. "Kemijski sastav i protuvirusno djelovanje hidrolata vrste Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. na virus mozaika duhana." Master's thesis, University of Split, University of Split, Faculty of science, 2018. https://urn.nsk.hr/urn:nbn:hr:166:076828

Mandić, Nela. "Kemijski sastav i protuvirusno djelovanje hidrolata vrste Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. na virus mozaika duhana." Master's thesis, University of Split, University of Split, Faculty of science, 2018. https://urn.nsk.hr/urn:nbn:hr:166:076828

Mandić, N. (2018). 'Kemijski sastav i protuvirusno djelovanje hidrolata vrste Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. na virus mozaika duhana', Master's thesis, University of Split, University of Split, Faculty of science, accessed 22 March 2019, https://urn.nsk.hr/urn:nbn:hr:166:076828

Mandić N. Kemijski sastav i protuvirusno djelovanje hidrolata vrste Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. na virus mozaika duhana [Master's thesis]. Split: University of Split, University of Split, Faculty of science; 2018 [cited 2019 March 22] Available at: https://urn.nsk.hr/urn:nbn:hr:166:076828

N. Mandić, "Kemijski sastav i protuvirusno djelovanje hidrolata vrste Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. na virus mozaika duhana", Master's thesis, University of Split, University of Split, Faculty of science, Split, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:166:076828

Metadata
TitleKemijski sastav i protuvirusno djelovanje hidrolata vrste Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. na virus mozaika duhana
Title (english)Chemical composition and antiviral activity of hydrolate of Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. on tobacco mosaic virus
AuthorNela Mandić
MentorElma Vuko (mentor)
Committee memberElma Vuko (predsjednik povjerenstva)
Committee memberValerija Dunkić (član povjerenstva)
Committee memberMirko Ruščić (član povjerenstva)
GranterUniversity of Split
University of Split, Faculty of science
(Department of Biology)
Split
Defense date and country2018-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Vrsta Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. (Clusiaceae) aromatična je biljna vrsta čija je ljekovitost od davnina poznata u narodnoj medicini. Iz biljnog materijala je izdvojen hidrolat kao nusprodukt vodene destilacije pri izolaciji eteričnog ulja. Analiza kemijskog sastava hidrolata provedena je primjenom tekućinske kromatografije visoke djelotvornosti (HPLC). Najzastupljenije komponente u sastavu hidrolata su 3,4-dihidroksibenzojeva kiselina (22,66µg/mL), apigenin (2,57µg/mL) i pinorezinol (2,46µg/mL). Protuvirusno djelovanje hidrolata na virus mozaika duhana ispitano je na biljkama koje su lokalni i sistemični domaćini ovog virusa. Pred tretman biljaka hidrolatom smanjuje broj virusnih lezija na listovima lokalnih domaćina i koncentraciju virusa u sistemično inficiranim biljkama. Dobiveni rezultati ukazuju da tretman hidrolatom vrste H. perforatum ssp. veronense jača otpornost biljaka prema virusnoj infekciji te da hidrolati aromatičnih biljnih vrsta imaju potencijal za razvoj prirodnih protufitovirusnih preparata.
Abstract (english)
Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb. (Clusiaceae) is an aromatic plant species well known in folk medicine. Hydrolate was isolated from plant material and an analysis of it's chemical composition was carried out using high performance liquid chromatography (HPLC). The main components of the hydrolate are 3,4-dihydroxybenzoic acid (22.66µg/mL), apigenin (2.57µg/mL) and pinorezinol (2.46µg/mL). Antiviralactivity of the hydrolate was tested on plants that are local and systemic hosts of tobacco mosaic virus. Pretreatment by hydrolate reduced the number of lesions on the leaves of local host plants as well as the virus concentration in systemically infected plants. The results indicate that treatment by hydrolate of H. perforatum ssp. veronense increases the plants resistance to viral infection and that hydrolates of aromatic plant species have the potential for development of natural antiphytoviral preparations.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:166:076828
Study programmeTitle: Biologija i kemija; smjerovi: nastavnički
Study programme type: sveučilišni
Study level: diplomski
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourcetext
File originBorn digital
Access conditionsRestricted access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
Created on2018-11-05 15:59:41