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Synthesis of Recombinant Human Insulin
Nina Žarković (2018)
University of Split
University of Split, Faculty of science
Department of Biology
Cite this document...

Žarković, N. (2018). Sinteza rekombinantnog humanog inzulina (Undergraduate thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:180052

Žarković, Nina. "Sinteza rekombinantnog humanog inzulina." Undergraduate thesis, University of Split, University of Split, Faculty of science, 2018. https://urn.nsk.hr/urn:nbn:hr:166:180052

Žarković, Nina. "Sinteza rekombinantnog humanog inzulina." Undergraduate thesis, University of Split, University of Split, Faculty of science, 2018. https://urn.nsk.hr/urn:nbn:hr:166:180052

Žarković, N. (2018). 'Sinteza rekombinantnog humanog inzulina', Undergraduate thesis, University of Split, University of Split, Faculty of science, accessed 27 March 2019, https://urn.nsk.hr/urn:nbn:hr:166:180052

Žarković N. Sinteza rekombinantnog humanog inzulina [Undergraduate thesis]. Split: University of Split, University of Split, Faculty of science; 2018 [cited 2019 March 27] Available at: https://urn.nsk.hr/urn:nbn:hr:166:180052

N. Žarković, "Sinteza rekombinantnog humanog inzulina", Undergraduate thesis, University of Split, University of Split, Faculty of science, Split, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:166:180052

Metadata
TitleSinteza rekombinantnog humanog inzulina
Title (english)Synthesis of Recombinant Human Insulin
AuthorNina Žarković
MentorŽeljana Fredotović (mentor)
Committee memberŽeljana Fredotović (predsjednik povjerenstva)
Committee memberElma Vuko (član povjerenstva)
Committee memberIvica Šamanić (član povjerenstva)
GranterUniversity of Split
University of Split, Faculty of science
(Department of Biology)
Split
Defense date and country2018-09-11, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Rekombinantni humani inzulin prvi je humani protein proizveden rekombinantnom DNA tehnologijom. Proces proizvodnje inzulina podrazumijeva sintezu gena, ugradnju gena u vektorsku molekulu, transformaciju stanice koja služi kao ekspresijski sustav i pročišćavanje dobivenog produkta. Standardni ekspresijski sustavi korišteni u industrijskoj proizvodnji su bakterija Escherichia coli i kvasac Saccharomyces cerevisiae. Rast broja dijabetičara i razvoj alternativnih načina terapije povećavaju potrebu za inzulinom koju trenutni mehanizmi proizvodnje ne mogu zadovoljiti. Intenzivno se istražuju složeniji ekspresijski sustavi (biljke i životinje) te mogućnost primjene tih sustava na proizvodnju velikih razmjera.
Abstract (english)
Recombinant human insulin was the first human protein made by recombinant DNA technology. The process of manufacturing insulin consists of gene synthesis, incorporation of the gene into a vector molecule, transformation of cells used as expression systems and finally protein purification. Standard expression systems in industrial protein manufacturing include the bacteria Escherichia coli and the yeast Saccharomyces cerevisiae. The incidence of diabetes is increasing rapidly along with alternative delivery systems development. The incerasing demand for insulin is barely met by current manufacturing capacities. More complex expression systems (plants, animals) and their large-scale manufacturing possibilities are being explored.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:166:180052
Study programmeTitle: Biologija i kemija
Study programme type: sveučilišni
Study level: preddiplomski
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije i kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije i kemije)
Type of resourcetext
File originBorn digital
Access conditionsRestricted access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
Created on2018-10-29 16:30:15