The urban flora of Imotski contains 321 types of vascular plants investigated on 9 anthropogenic ruderal habitats. The most populated family in the flora are Asteraceae (48.5%) and Poaceae (43%). The dominant lifestyle in the flora of Imotski are terophytes (37.5%), and flora elements are dominated by plants with a wide spread (26.6%), Mediterranean flora (23%) and cultivated and adventive plants (22.4%). Dominant plants on ruderal habitats are Ailanthus altissima. Polygonum aviculare, Cynodon dactylon, Portulaca oleracea, Setaria viridis, Chenopodium album, Conyza canadensis ... In the total flora 11% of the species belongs to the alohton adventive plants (neophytes), which are constantly spreading and naturalizing into the composition of natural and anthropogenic habitats. The largest number of neophyte originates from the American continent 22 (63%). One of the most invasive species in the flora of Imotski and flora of Croatia is either Ailanthus altissima, which is spread almost in all the habitats of the urban flora of Imotski. In the explored flora of Imotski, two endemic species belonging to the Illyrian Adriatic endemic plants and 4 endangered plant species belonging to endangered species were identified.