Download
PDF 1.4 MB
master's thesis
24   13
Seeing different lateral organization in membrane bilayer made of various ternary mixtures of phospholipids, sphingomyelin and cholesterol by fluorescent microscopy
Gorana Glavurtić (2016)
University of Split
University of Split, Faculty of science
Department of Physics
Cite this document...

Glavurtić, G. (2016). Seeing different lateral organization in membrane bilayer made of various ternary mixtures of phospholipids, sphingomyelin and cholesterol by fluorescent microscopy (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:842002

Glavurtić, Gorana. "Seeing different lateral organization in membrane bilayer made of various ternary mixtures of phospholipids, sphingomyelin and cholesterol by fluorescent microscopy." Master's thesis, University of Split, University of Split, Faculty of science, 2016. https://urn.nsk.hr/urn:nbn:hr:166:842002

Glavurtić, Gorana. "Seeing different lateral organization in membrane bilayer made of various ternary mixtures of phospholipids, sphingomyelin and cholesterol by fluorescent microscopy." Master's thesis, University of Split, University of Split, Faculty of science, 2016. https://urn.nsk.hr/urn:nbn:hr:166:842002

Glavurtić, G. (2016). 'Seeing different lateral organization in membrane bilayer made of various ternary mixtures of phospholipids, sphingomyelin and cholesterol by fluorescent microscopy', Master's thesis, University of Split, University of Split, Faculty of science, accessed 27 March 2019, https://urn.nsk.hr/urn:nbn:hr:166:842002

Glavurtić G. Seeing different lateral organization in membrane bilayer made of various ternary mixtures of phospholipids, sphingomyelin and cholesterol by fluorescent microscopy [Master's thesis]. Split: University of Split, University of Split, Faculty of science; 2016 [cited 2019 March 27] Available at: https://urn.nsk.hr/urn:nbn:hr:166:842002

G. Glavurtić, "Seeing different lateral organization in membrane bilayer made of various ternary mixtures of phospholipids, sphingomyelin and cholesterol by fluorescent microscopy", Master's thesis, University of Split, University of Split, Faculty of science, Split, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:166:842002

Metadata
TitleSeeing different lateral organization in membrane bilayer made of various ternary mixtures of phospholipids, sphingomyelin and cholesterol by fluorescent microscopy
Title (croatian)Proučavanje lateralne organizacije membranskog dvosloja načinjenih od različitih trokomponentnih smjesa fosfolipida, sfingomijelina i kolesterola uz primjenu fluorescentne mikroskopije
AuthorGorana Glavurtić
MentorMarija Raguž (mentor)
Committee memberMarija Raguž (predsjednik povjerenstva)
Committee memberJasna Puizina (član povjerenstva)
Committee memberAnte Bilušić (član povjerenstva)
GranterUniversity of Split
University of Split, Faculty of science
(Department of Physics)
Split
Defense date and country2016-09-14, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract
Vesicle is lipid membrane bilayer which simulates the cell membrane in terms of its dimension and biophysical properties. The basic building blocks of vesicle is lipid, which consists of hydrophilic “head” and hydrophobic “tail” and forms lipid bilayer in aqueous environment. Lipid bilayer is heterogeneous and it consists of many different kinds of lipid. The most abundant are phospholipid, sphingolipids and sterols. Experiment was performed for different three-component mixture of phosphatidylcholine, sphingomyelin (SM) and cholesterol (Chol). Four different kinds of phosphatidylcholine were chosen (1-palmitoyl-2oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2- dimyristoyl-sn-glycero-3phophocholine (DMPC)). Chosen phosphatidylcholines have different length and degree of saturation of their acyl chains. Different membrane domains are formed depends on its lipid composition. All molar ratios of vesicle lipid components were chosen based on previously reported phase diagram for ternary mixture of SM, POPC and Chol. Different membrane domains, including liquid-ordered (Lo) and liquid-disordered (Ld), will be form depends on concentration of cholesterol and other lipids in vesicle. Results obtained for vesicles made of SM, POPC and Chol confirmed literature data. Depends on length and degree of saturation of phospholipid fatty acid different lipid domains were experimentally detected for the same cholesterol and SM molar content. The number of formed domains in vesicles that contain DSPC, DPPC and DMPC depends on phase transition temperature, Tm.
Abstract (croatian)
Vezikule predstavljaju lipidne membranske dvosloje koje svojim dimenzijama i biofizikalnom svojstvima modeliraju staničnu membranu. Osnovna gradivna jedinica vezikule je lipid, koji se sastoji od hidrofilne “glave” i hidrofobnog “repa” te omogućuje stvaranje lipidnog dvosloja u vodenoj otopini. Lipidni dvosloj je heterogen i sastoji se od različitih vrsta lipida. Najviše zastupljeni su fosfolipidi, sfingolipidi i steroli. Eksperiment je proveden za različite trokomponentne smjese fosfatidilkolina, sfingomijelina i kolesterola. Odabrali smo četiri različite vrste fosfatidilkolina (sa dvije stearinske, palmitinske i miristrinske masne kiseline kao i sa kombinacijom oleinske i palmitinske kiseline). Odabrani fosfatidilkolini su imali različitu duljinu i stupanj zasićenosti masnih kiselina. Različite lipidne domene se formiraju u vezikuli ovisno o njenom molekularnom sastavu. Molarni udjeli lipidnih komponenti u vezikuli su odabrani na osnovu prethodno objavljenih faznih dijagrama za trokomponentnu smjesu sfingomijelina, fosfatidilkolina sa kombinacijom oleinske i palmitinske masne kiseline i kolesterola. Promjenom lipidnog sastava (koncentracije kolesterola i ostalih lipida u vezikuli) formirat će se različite domene, uključujući uređene (Lo) i neuređene (Ld) tekuće domene. Dobiveni rezultati za vezikule načinjene od sfingomijelina, fosfatidilkolina sa kombinacijom oleinske i palmitinske masne kiseline i kolesterola su potvrdili rezultate objavljene u literaturi. Ovisno o dužini i stupnju zasićenosti masnih kiselina fosfatidilkolina eksperimentalno su detektirane različite lipidne domene za jednaki molarni udio kolesterola i sfingomijelina. Broj formiranih domena u vezikulama koje sadrže fosfatidilkoline sa zasićenim masnim kiselinama (sa dvije stearinske, palmitinske i miristrinske masne kiseline) ovisi o temperaturi faznog prijelaza, Tm.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:166:842002
Study programmeTitle: Fizika; smjerovi: Nastavnički, Astrofizika, Biofizika, Fizika okoliša, Računarska fizika
Study programme type: sveučilišni
Study level: diplomski
Academic / professional title: magistar/magistra fizike (magistar/magistra fizike)
Type of resourcetext
File originBorn digital
Access conditionsOpen access
Terms of usehttp://creativecommons.org/licenses/by-nc-sa/4.0/
Created on2017-10-03 15:34:31