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Myxothiazol induced reverse reaction in Rhodobacter capsulatus cytochrome bc1 mutants
Tomica Kralj (2015)
University of Split
University of Split, Faculty of science
Department of Physics
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Kralj, T. (2015). Myxothiazol induced reverse reaction in Rhodobacter capsulatus cytochrome bc1 mutants (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:844393

Kralj, Tomica. "Myxothiazol induced reverse reaction in Rhodobacter capsulatus cytochrome bc1 mutants." Master's thesis, University of Split, University of Split, Faculty of science, 2015. https://urn.nsk.hr/urn:nbn:hr:166:844393

Kralj, Tomica. "Myxothiazol induced reverse reaction in Rhodobacter capsulatus cytochrome bc1 mutants." Master's thesis, University of Split, University of Split, Faculty of science, 2015. https://urn.nsk.hr/urn:nbn:hr:166:844393

Kralj, T. (2015). 'Myxothiazol induced reverse reaction in Rhodobacter capsulatus cytochrome bc1 mutants', Master's thesis, University of Split, University of Split, Faculty of science, accessed 22 March 2019, https://urn.nsk.hr/urn:nbn:hr:166:844393

Kralj T. Myxothiazol induced reverse reaction in Rhodobacter capsulatus cytochrome bc1 mutants [Master's thesis]. Split: University of Split, University of Split, Faculty of science; 2015 [cited 2019 March 22] Available at: https://urn.nsk.hr/urn:nbn:hr:166:844393

T. Kralj, "Myxothiazol induced reverse reaction in Rhodobacter capsulatus cytochrome bc1 mutants", Master's thesis, University of Split, University of Split, Faculty of science, Split, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:166:844393

Metadata
TitleMyxothiazol induced reverse reaction in Rhodobacter capsulatus cytochrome bc1 mutants
Title (croatian)Povratna reakcija u Rhodobacter capsulatus citokrom bc1 mutantima inducirana inhibitorom miksotiazolom
AuthorTomica Kralj
MentorMarija Raguž (mentor)
Committee memberMarija Raguž (predsjednik povjerenstva)
Committee memberStjepan Orhanović (član povjerenstva)
Committee memberAnte Bilušić (član povjerenstva)
GranterUniversity of Split
University of Split, Faculty of science
(Department of Physics)
Split
Defense date and country2015-09-04, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract
Cytochrome bc1 (also known as complex III) is an enzyme of electron transport chain and is found in both bacteria and mitochondria. It has an important role in cellular respiration as it contributes to the generation of the proton motive force which is later used for ATP synthesis. It does this by coupling oxidation and reduction of quinones (commonly called Q-cycle) to proton translocation across inner mitochondrial or bacterial plasma membrane. Stable semiquinone is formed in one of the catalytic sites of cytochrome bc1 (Qi site) during its enzymatic activity. We analysed mutants of cytochrome bc1 that were thought to have even more stable semiquinone than wild type due to steric hinderance introduced at the Qi site. Experiments performed on Rhodobacter capsulatus mutants indicate that during reverse reaction hemes bH get reduced more than in the same reaction in wild type. However, despite this no semiquinone signal could be detected at Qi site in those mutants.
Abstract (croatian)
Citokrom bc1 (također poznat i kao kompleks III) je enzim transportnog lanca elektrona te ga se može naći u bakterijama i mitohondrijima. Ima važnu ulogu u staničnom disanju, budući da doprinosi stvaranju elektrokemijskog gradijenta protona koji se kasnije koristi za sintezu ATP-a. To čini tako da spaja oksidaciju i redukciju kinona (zajednički nazvane Q ciklus) sa premještanjem protona preko unutarnje membrane mitohondrija ili plazma membrane bakterija. Tijekom ove enzimatske aktivnosti, stabilni semikinon se stvara u jednom od katalitičkih mjesta citokroma bc1 (Qi mjesto). Analizirali smo mutante citokroma bc1 za koje se smatralo da bi mogli imati još stabilniji semikinon nego divlji tip zbog prostornih smetnji induciranih u Qi mjestu. Pokusi rađeni na Rhodobacter capsulatus mutantima ukazuju da su tijekom povratne reakcije hemovi bH reducirani više nego tijekom iste reakcije u divljem tipu. Ipak, unatoč tome nije bilo moguće opaziti nikakav signal semikinona u Qi mjestu u tim mutantima.
Keywords
Languageenglish
URN:NBNurn:nbn:hr:166:844393
Study programmeTitle: Fizika; smjerovi: Nastavnički, Astrofizika, Biofizika, Fizika okoliša, Računarska fizika
Study programme type: sveučilišni
Study level: diplomski
Academic / professional title: magistar/magistra fizike (magistar/magistra fizike)
Type of resourcetext
File originBorn digital
Access conditionsOpen access
Terms of usehttp://creativecommons.org/licenses/by-nc-sa/4.0/
Public noteKljučne riječi unio urednik.
Created on2017-06-26 15:40:01