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master's thesis
Effects of Electromagnetic Stimulation and Rapamycin on Morphology and Autophagy of In-vitro Cultured Spiral Ganglion Neurons
2024. urn:nbn:hr:166:148062
Radišić, Dubravko
University of Split
Faculty of Science

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Radišić, D. (2024). Effects of Electromagnetic Stimulation and Rapamycin on Morphology and Autophagy of In-vitro Cultured Spiral Ganglion Neurons (Master's thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:148062

Radišić, Dubravko. "Effects of Electromagnetic Stimulation and Rapamycin on Morphology and Autophagy of In-vitro Cultured Spiral Ganglion Neurons." Master's thesis, University of Split, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:166:148062

Radišić, Dubravko. "Effects of Electromagnetic Stimulation and Rapamycin on Morphology and Autophagy of In-vitro Cultured Spiral Ganglion Neurons." Master's thesis, University of Split, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:166:148062

Radišić, D. (2024). 'Effects of Electromagnetic Stimulation and Rapamycin on Morphology and Autophagy of In-vitro Cultured Spiral Ganglion Neurons', Master's thesis, University of Split, Faculty of Science, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:166:148062

Radišić D. Effects of Electromagnetic Stimulation and Rapamycin on Morphology and Autophagy of In-vitro Cultured Spiral Ganglion Neurons [Master's thesis]. Split: University of Split, Faculty of Science; 2024 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:166:148062

D. Radišić, "Effects of Electromagnetic Stimulation and Rapamycin on Morphology and Autophagy of In-vitro Cultured Spiral Ganglion Neurons", Master's thesis, University of Split, Faculty of Science, Split, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:166:148062

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Metadata
TitleEffects of Electromagnetic Stimulation and Rapamycin on Morphology and Autophagy of In-vitro Cultured Spiral Ganglion Neurons
Title (croatian)Utjecaj elektromagnetske stimulacije i rapamicina na morfologiju i autofagiju in-vitro uzgojenih kultura neurona spiralnog ganglija
AuthorDubravko Radišić
MentorDamir Kovačić (mentor)
Committee memberDamir Kovačić (predsjednik povjerenstva)
Committee memberMija Marinković (član povjerenstva)
Committee memberIvana Weber (član povjerenstva)
GranterUniversity of Split
Faculty of Science
Split
Defense date and country2024-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract
Spiral ganglion neurons (SGN) transmit auditory signals from sensory-receptive hair cells to the cochlear nucleus in the brain. Damage to hair cellstypically leads to degeneration of SGN and represents one of the leading causes of sensorineural hearing loss (SNHL) worldwide. The effectiveness of the most successful auditory prosthetic, the cochlear implant, in treating SNHL depends on the number and health of preserved SGNs. In this study, we assessed whether autophagy induction via rapamycin, a crucial cellular process responsible for maintaining cellular homeostasis and health, can be combined with a pulsed electromagnetic field (EMF) stimulation to strengthen the growth and health of neonatal SGNs, cultured in-vitro. The results showed that EMF stimulation plays a role in autophagy and that combining rapamycin and EMF stimulation strengthens the SGN outgrowth significantly. This effect and the impact on morphological characteristics and cellular health varied sensitively with different concentrations of rapamycin. This study demonstrates that modulation of autophagic activity, in combination with other methods, may be utilized as a potential therapeutic strategy to enhance the efficacy of cochlear implants.
Abstract (croatian)
Neuroni spiralnog ganglija (NSG) su primarni auditorni neuroni odgovorni za prijenos auditornog signala iz osjetnih stanica s dlačicama do kohlearne jezgre u mozgu. Njihova degeneracija, uz oštećenje osjetnih stanica s dlačicama, jedan je od glavnih uzroka zamjedbenog (senzoneuralnog) gubitka sluha (SNHL). Efikasnost najuspješnijeg uređaja medicinske bionike, kohlearnog implantata (umjetne pužnice), ovisi o broju i funkcionalnom stanju NSG. U ovome radu promotrili smo utjecaj primjene elektromagnetskog polja i indukcije autofagije preko primjene rapamicina, esencijalnog staničnog procesa odgovornog za održavanje homeostaze i zdravlja stanica, kao potencijalnu kombiniranu metodu promoviranja većeg rasta i zdravlja neonatalnih NSG-ova. Rezultati su pokazali da EMF stimulacija utječe na autofagiju, te da kombinacija rapamicina i EMF stimulacije može postići značajno veći rast NSG-ova. Ovaj efekt, kao i utjecaj na morfološke karakteristike i stanično zdravlje, varirao je srazličitim koncentracijama rapamicina. Ovaj rad pokazuje da se modulacija autofagijske aktivnosti u kombinaciji sa drugim metodama može koristiti kao potencijalna terapeutska strategija za pobolšanje efikasnosti umjetne pužnice.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:166:148062
Study programmeTitle: Physics; specializations in: specialization in Education, specialization in Astrophysics and Elementary Particle Physics, specialization in Biophysics, specialization in Environmental Physics, specialization in Computational Physics
Course: specialization in Biophysics
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra fizike (sveučilišni/a magistar/magistra fizike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-10-25 12:40:47