prikaz prve stranice dokumenta Bakterijska rezistencija na antibiotike Klebsiella i Enterobacter izolata iz uređaja za otpadne vode u Hrvatskoj
Embargo period 2027-09-25
master's thesis
Bakterijska rezistencija na antibiotike Klebsiella i Enterobacter izolata iz uređaja za otpadne vode u Hrvatskoj
2024. urn:nbn:hr:166:920838
Župić, Sara
University of Split
Faculty of Science

Cite this document

Župić, S. (2024). Bakterijska rezistencija na antibiotike Klebsiella i Enterobacter izolata iz uređaja za otpadne vode u Hrvatskoj (Master's thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:920838

Župić, Sara. "Bakterijska rezistencija na antibiotike Klebsiella i Enterobacter izolata iz uređaja za otpadne vode u Hrvatskoj." Master's thesis, University of Split, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:166:920838

Župić, Sara. "Bakterijska rezistencija na antibiotike Klebsiella i Enterobacter izolata iz uređaja za otpadne vode u Hrvatskoj." Master's thesis, University of Split, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:166:920838

Župić, S. (2024). 'Bakterijska rezistencija na antibiotike Klebsiella i Enterobacter izolata iz uređaja za otpadne vode u Hrvatskoj', Master's thesis, University of Split, Faculty of Science, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:166:920838

Župić S. Bakterijska rezistencija na antibiotike Klebsiella i Enterobacter izolata iz uređaja za otpadne vode u Hrvatskoj [Master's thesis]. Split: University of Split, Faculty of Science; 2024 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:166:920838

S. Župić, "Bakterijska rezistencija na antibiotike Klebsiella i Enterobacter izolata iz uređaja za otpadne vode u Hrvatskoj", Master's thesis, University of Split, Faculty of Science, Split, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:166:920838

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Metadata
TitleBakterijska rezistencija na antibiotike Klebsiella i Enterobacter izolata iz uređaja za otpadne vode u Hrvatskoj
Title (english)Bacterial resistance to antibiotics in Klebsiella and Enterobacter isolates from wastewater treatment plants in Croatia
AuthorSara Župić
MentorAna Maravić (mentor)
MentorMarija Kvesić Ivanković (pomoć pri izradi, neposredni voditelj)
Committee memberAna Maravić (predsjednik povjerenstva)
Committee memberIvica Šamanić (član povjerenstva)
Committee memberMarija Kvesić Ivanković (član povjerenstva)
GranterUniversity of Split
Faculty of Science
Split
Defense date and country2024-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Cilj ovog diplomskog rada je izolirati karbapenem-rezistentne Klebsiella i Enterobacter izolate iz uzoraka otpadne vode iz uređaja za otpadne vode (UPOV-i) grada Splita i morske vode u blizini pripadajućih podmorskih ispusta u srednjem Jadranu, te testirati njihovu fenotipsku osjetljivost na antibiotike. Određena je razina fekalnog onečišćenja u uzorcima te je 108 izolata identificirano do vrste i ispitana njihova fenotipska osjetljivost na 16 antibiotika metodom disk-difuzije na Mueller-Hinton agaru. Najveći broj izolata pokazao je rezistenciju na cefoksitin (97,22%), piperacilin (90,74%), aztreonam (84,26%) i meropenem (79,63%), dok je najmanja učestalost rezistencije zabilježena na trimetoprim-sulfametoksazol (17,59%). Rezultati pokazuju visok postotak rezistencije na različite klase antibiotika, odnosno višestruko rezistentni fenotip izolata i moguću prisutnost karbapenemaza. Također, obzirom da UPOV Katalinića Brig obrađuje i otpadne vode iz KBC-a Split, mogući su rizici širenja bolničkih rezistentnih sojeva u morski okoliš, što potvrđuje potrebu za daljnjim praćenjem njihove učestalosti te nastavkom istraživanja u smjeru određivanja genotipa rezistencije ovih oportunističkih patogena kritičnog prioriteta.
Abstract (english)
The aim of this thesis was to isolate carbapenem-resistant Klebsiella and Enterobacter isolates from wastewater samples from wastewater treatment plants (WWTPs) of the city of Split and seawater from the adjacent submarine outlets in the central Adriatic, and to test their phenotypic sensitivity to antibiotics. The level of fecal contamination in the samples was determined, and 108 isolates were identified to species and their phenotypic sensitivity to 16 antibiotics was tested using the disk-diffusion method on Mueller-Hinton agar. The largest number of isolates showed resistance to cefoxitin (97.22%), piperacillin (90.74%), aztreonam (84.26%) and meropenem (79.63%), while the lowest frequency of resistance was recorded against trimethoprim-sulfamethoxazole (17.59%). The results show a high percentage of resistance to different classes of antibiotics, i.e. the multiple resistant phenotype of the isolates and the possible presence of carbapenemases. Also, considering that UPOV Katalinića Brig also processes wastewater from University Hospital Split, there are possible risks of the spread of hospital resistant strains into the marine environment, which confirms the need for further monitoring of their frequency and the continuation of research in the direction of determining the resistance genotype of these WHO critical-priority opportunistic pathogens.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:166:920838
Study programmeTitle: Biology and Chemistry; specializations in: specialization in Education
Course: specialization in Education
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra edukacije biologije i kemije (sveučilišni/a magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2027-09-25
Terms of useLicence In copyright icon
Created on2024-10-23 10:05:48