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master's thesis
Synthesis, structural studies, molecular modeling and characterization of helminth antimicrobial peptides
2022. urn:nbn:hr:166:228687
Čopac, Roko
University of Split
Faculty of Science
Department of Physics

Cite this document

Čopac, R. (2022). Synthesis, structural studies, molecular modeling and characterization of helminth antimicrobial peptides (Master's thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:228687

Čopac, Roko. "Synthesis, structural studies, molecular modeling and characterization of helminth antimicrobial peptides." Master's thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:228687

Čopac, Roko. "Synthesis, structural studies, molecular modeling and characterization of helminth antimicrobial peptides." Master's thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:228687

Čopac, R. (2022). 'Synthesis, structural studies, molecular modeling and characterization of helminth antimicrobial peptides', Master's thesis, University of Split, Faculty of Science, accessed 05 December 2024, https://urn.nsk.hr/urn:nbn:hr:166:228687

Čopac R. Synthesis, structural studies, molecular modeling and characterization of helminth antimicrobial peptides [Master's thesis]. Split: University of Split, Faculty of Science; 2022 [cited 2024 December 05] Available at: https://urn.nsk.hr/urn:nbn:hr:166:228687

R. Čopac, "Synthesis, structural studies, molecular modeling and characterization of helminth antimicrobial peptides", Master's thesis, University of Split, Faculty of Science, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:166:228687

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Metadata
TitleSynthesis, structural studies, molecular modeling and characterization of helminth antimicrobial peptides
Title (croatian)Sinteza,strukturne studije, molekularno modeliranje i karakterizacija antimikrobnih peptida u plošnjaka
AuthorRoko Čopac
MentorLarisa Zoranić (mentor)
MentorTomislav Rončević (pomoć pri izradi, neposredni voditelj)
MentorAndrea Caporale https://orcid.org/0000-0002-7827-3960 (pomoć pri izradi, neposredni voditelj)
MentorSabrina Pacor https://orcid.org/0000-0001-8043-6725 (pomoć pri izradi, neposredni voditelj)
Committee memberLarisa Zoranić (predsjednik povjerenstva)
Committee memberTomislav Rončević (član povjerenstva)
Committee memberŽeljka Sanader Maršić (član povjerenstva)
Committee memberLucija Krce (član povjerenstva)
Committee memberIvica Šamanić (član povjerenstva)
GranterUniversity of Split
Faculty of Science
(Department of Physics)
Split
Defense date and country2022-03-18, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Biophysics and Medical Physics
Abstract
Antimicrobial resistance, which can be partially attributed to irresponsible and improper use of antibiotics, remains a major concern worldwide in recent decades. One of the potential alternatives to classical antibiotic treatment are antimicrobial peptides (AMPs) - structurally different (macro)molecules with direct antimicrobial and/or immunomodulatory properties. Helminths, a group of parasitic organisms, successfully co-exist in bacteria-rich surroundings during their life cycle, and among the possible mechanisms of defense of these organisms are likely be AMPs. By exploring the genomes of several helminth species, 31 AMPs were obtained, after which a bioinformatics analysis was performed and 3 were selected for further characterization. Moreover, several N- and C-terminal fragments of a peptide identified in Taenia solium were synthesized to conduct structure-activity studies. All selected peptides, as well as the fragments, were characterized by structural, antimicrobial, and cytotoxic tests, and modeled by molecular dynamics simulations. Most of the peptides showed broad-spectrum antimicrobial activity, with (mostly) acceptable levels of toxicity, which warrants further mode-of-action studies, and development for possible biomedical applications.
Abstract (croatian)
Rezistencija bakterija na antibiotike, za koju je djelomično zaslužna neodgovorna i pretjerana uporaba, gorući je problem diljem svijeta u zadnjim desetljećima. Kao potencijalna alternativa klasičnim antibioticima nameću se antimikrobni peptidi (AMP), strukturno različite makromolekule koje imaju direktnu antimikrobnu i/ili imunomodulatornu aktivnost. Plošnjaci, skupina nametničkih organizma, uspješno ko-egzistira u okolini (najčešće) bogatoj bakterijama, a jedan od mogućih mehanizama obrane ovih organizama su AMP-i. Analizom genoma većeg broja plošnjaka, identificirali smo 31 AMP, te nakon detaljne bioinformatičke analize odabrali tri peptida za daljine istraživanje. Istovremeno, u cilju ispitivanja utjecaja pojedinih segmenata peptidne sekvence na aktivnost i mehanizam djelovanja, sintetizirani su N- i C-fragmenti peptida identificiranog u Taenia solium. Svi odabrani peptidi, kao i fragmenti, karakterizirani su putem strukturalnih, antimikrobnih i citotoksičnih ispitivanja, te istraženi na molekularnoj razini korištenjem simulacija molekularne dinamike. Većina peptidi pokazuje antimikrobnu aktivnost širokog spektra, dok je toksičnost često u prihvatljivim okvirima, što otvara vrata daljnjim ispitivanjima mehanizama djelovanja, kao i potencijalnoj biomedicinskoj uporabi.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:166:228687
Study programmeTitle: Physics; specializations in: major in teaching, major in Astrophysics, major in Biophysics, major in Enviromental physics, major in Computational physics, major in Astrophysics and physics of elemental particles
Course: major in Biophysics
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra fizike (magistar/magistra fizike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-03-18
Terms of useLicence In copyright icon
Created on2022-03-24 13:16:25