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master's thesis
Heterologna ekspresija i pročišćavanje transkripcijskog faktora QacR iz bakterije Escherichia coli
2021. urn:nbn:hr:166:170369
Vidaković, Josip
University of Split
University of Split, Faculty of science
Department of Chemistry

Cite this document

Vidaković, J. (2021). Heterologna ekspresija i pročišćavanje transkripcijskog faktora QacR iz bakterije Escherichia coli (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:170369

Vidaković, Josip. "Heterologna ekspresija i pročišćavanje transkripcijskog faktora QacR iz bakterije Escherichia coli." Master's thesis, University of Split, University of Split, Faculty of science, 2021. https://urn.nsk.hr/urn:nbn:hr:166:170369

Vidaković, Josip. "Heterologna ekspresija i pročišćavanje transkripcijskog faktora QacR iz bakterije Escherichia coli." Master's thesis, University of Split, University of Split, Faculty of science, 2021. https://urn.nsk.hr/urn:nbn:hr:166:170369

Vidaković, J. (2021). 'Heterologna ekspresija i pročišćavanje transkripcijskog faktora QacR iz bakterije Escherichia coli', Master's thesis, University of Split, University of Split, Faculty of science, accessed 17 May 2022, https://urn.nsk.hr/urn:nbn:hr:166:170369

Vidaković J. Heterologna ekspresija i pročišćavanje transkripcijskog faktora QacR iz bakterije Escherichia coli [Master's thesis]. Split: University of Split, University of Split, Faculty of science; 2021 [cited 2022 May 17] Available at: https://urn.nsk.hr/urn:nbn:hr:166:170369

J. Vidaković, "Heterologna ekspresija i pročišćavanje transkripcijskog faktora QacR iz bakterije Escherichia coli", Master's thesis, University of Split, University of Split, Faculty of science, Split, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:166:170369

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Metadata
TitleHeterologna ekspresija i pročišćavanje transkripcijskog faktora QacR iz bakterije Escherichia coli
Title (english)Heterologus expression and purification of transcription factor QacR from bacteria Escherichia coli
AuthorJosip Vidaković
MentorMatilda Šprung (mentor)
MentorDoris Crnčević (pomoć pri izradi, neposredni voditelj)
Committee memberMatilda Šprung (predsjednik povjerenstva)
Committee memberDoris Crnčević (član povjerenstva)
Committee memberStjepan Orhanović (član povjerenstva)
GranterUniversity of Split
University of Split, Faculty of science
(Department of Chemistry)
Split
Defense date and country2021-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Transkripcijski faktor QacR regulatorni je protein povezan s mehanizmom rezistencije bakterije Staphylococcus aureus na mnoge antibiotike, dezinficijense te kvaterne amonijeve soli. Ovaj represorski protein kontrolira ekspresiju gena qacA. Ekspresijom gena qacA povećava se koncentracija proteina QacA odgovornog za mehanizam rezistencije. Ovaj protein predstavlja „pumpu“ koja iz stanice izbacuje toksične spojeve. Upravo rezistencija na mnoge antibiotike te velika rasprostranjenost meticilin rezisentnog S. aureus (MRSA) potaknula je veliki interes za istraživanje ovih proteina. U ovom radu plazmid s ukloniranim genom qacR ugrađen je u kompetentne stanice Escherichia coli. Eksprimirani QacR protein pročišćen je afinitetnim kromatografijom te vizualiziran na poliakrilamidnom gelu.
Abstract (english)
The transcription factor QacR is a regulatory protein associated with the mechanism of resistance of Staphylococcus aureus to many antibiotics, disinfectants, and quaternary salts. This repressor protein controls the expression of the qacA gene. Expression of this gene increases the concentration of QacA protein responsible for the resistance mechanism. This protein is a "pump" that expels toxic compounds from the cell. It is the resistance to many antibiotics and the high prevalence of methicillin-resistant S. aureus (MRSA) that has prompted great interest in research of these proteins. In this work, a plasmid containing qacR gene was inserted into competent Escherichia coli cells. The expressed QacR protein was purified by affinity chromatography and visualized on a polyacrylamide gel.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:166:170369
Study programmeTitle: Biology and Chemistry; specializations in: major in teaching
Course: major in teaching
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2021-10-22 10:26:34